But studies are showing that is not the case. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. A segment of the protein called the open reading frame-1, or ORF-1, then goes back into the nucleus, where it attaches to the vaccines mRNA and reverse transcribes into spike DNA. Differences in mRNA-1273 (Moderna) and BNT162b2 (Pfizer-BioNTech) SARS-CoV-2 vaccine immunogenicity among patients undergoing dialysis. Why liver cells and why the specific dose? Huh7, MeSH WebPfizer Vaccine Becomes DNA in Liver Cells. This has been the question our study aims to answer. Its precisely what health experts and fact-checkers said for more than a year could not occur. Sudden deaths of Canadian This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. They are not sure if it gets integrated in our DNA or not. 1 . Clipboard, Search History, and several other advanced features are temporarily unavailable. eCollection 2022. A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. For medical concerns, including decisions about vaccinations, medications and other treatments, you should always consult your physician or, in serious cases, seek immediate assistance from emergency personnel. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. Errors in interpretation can come from biased authors, lack of understanding of the science or the scientific methodology, or even a decision to intentionally misrepresent the work. part may be reproduced without the written permission. The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. Your email address is used only to let the recipient know who sent the email. Science X Daily and the Weekly Email Newsletter are free features that allow you to receive your favorite sci-tech news updates in your email inbox, New HIV mRNA vaccine study gets boost from COVID mRNA vaccine development, How gut bacteria can impact treatments for cancer, Human feces and urine contain a motherlode of health data: 'Smart toilets' detect daily fluctuations, serious disease, Multisite multiomic analysis reveals diverse resistance mechanisms in end-stage ovarian cancer, Review of data surrounding COVID vaccines and pregnant women suggest no increased risk of miscarriage, A possible way to prevent or treat lung damage associated with long COVID. Huang X, Kong N, Zhang X, Cao Y, Langer R, Tao W. Nat Med. Two people in their 60s who received the shot were diagnosed with Guillain-Barre syndrome, out of about 20,000 vaccine recipients who participated in Pfizers Phase 3 Clin Microbiol Infect. N. Engl. Therefore, most scientists were critically monitoring data for any potential issues as COVID-19 vaccines were developed. 2020;383:26032615. In the general population, the antibody response wanes over the course of time after two doses of the vaccination, but seems to be more Antibody levels were also measured in separate tests. doi: 10.1056/NEJMoa2027906. The whole process occurred rapidly, within six hours, concluded the study, which was published by the universitys Department of Clinical Sciences. 2022 Nov;28(11):2273-2287. doi: 10.1038/s41591-022-02061-1. It only presents the body with the instructions to build immunity.. (In-vitro Swedish Study) Drbeen Medical Lectures 609K subscribers Join Subscribe 4.3M views Streamed 11 Pfizer Vaccine Becomes DNA in Liver Cells. Indeed, about 8 percent of human DNA comes from viruses inserted into our genomes during evolution. YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. [(accessed on 22 February 2022)]. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. (Infographic). All quotes delayed a minimum of 15 minutes. Well do this by highlighting a few papers that caused fear, generated confusion, or spread misinformation by looking at the interpretation, what it was based on, and why it was not correct. It was assumed that the vaccines spike protein would remain mostly at the injection site and last up to several weeks like other proteins produced in the body. Because the study design does not necessarily reflect what happens in most human bodies, he said the findings cannot be extrapolated to make inferences about what would happen in human subjects. But that wasnt observed in this study. followers 129 videos. A new study from Lund University in Sweden on how the Pfizer-BioNTech COVID-19 vaccine affects human liver cells under experimental conditions, has been viewed more than 800,000 times in just over a week. The more virus in a sample, the fewer cycles it would take to detect RNA. The genetic material delivered by mRNA vaccines never enters the nucleus of your cells, the CDC said on its web page titled Myths and Facts about COVID-19 Vaccines.. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. Fax: +46 (0)46 222 47 20, Invoice address: Box 188, 221 00 Lund They certainly tell us something we did not know without the study, but it is important to understand the limitations of the data. Front Immunol. 2022 Dec 9;14(12):e32361. Lund Universitymagnus [dot] rasmussen [at] med [dot] lu [dot] se, Yang de Marinis Link to the publication in Molecular Biology: Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line. The result might be surprising, but it is also a bit surprising that such studies do not seem to have been carried out before. They have two things in common. In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage J Transl Autoimmun. Likewise, the Results section has five sentences. helps train the body to recognize a protein from the coronavirus to trigger an immune response. A new Swedish study published in MDPIfound that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact with your DNA in any way. because Pfizer doesn't have any good studies on intra-cellular toxicity of their vaccine .. it leaves behind genetic markers that may indicate a change in DNA of the The Swedish study suggests the vaccine is capable of becoming DNA, which is what the CDC claimed 3401 Civic Center Blvd. Parents PACK. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Cells that received no vaccine at all were used for control purposes. Accessibility Some viruses, like HIV, are known to be able to convert RNA to DNA and then incorporate that DNA into host cells genome, which is one thing that makes the illness difficult to treat. Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. This three-part series was aimed at describing several important aspects of science. As long as the spike protein can be detected on cell-derived membrane vesicles, the immune system will be attacking the cells that release these vesicles, they said. To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. doi: 10.1503/cmaj.211881. BNT162b2 mRNA levels in Huh7 cells treated with BNT162b2. CLAIM: A Swedish study shows that Pfizers COVID-19 vaccine changes recipients DNA. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the BNT162b2 injection. The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. They used different concentrations of the vaccine and Epub 2022 Nov 10. The authors said that they chose to study liver cells as Pfizer had reported that about 18% of the vaccine accumulated in the liver in 30 minutes after mice were injected. Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. Heres why: Lyons-Weiler J. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. doi: 10.1128/Spectrum.01162-21. The. The mRNA is converted to DNA as early as six hours after exposure to the vaccine. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. YDM: More research is needed. The results have been widely discussed across social media but the results have in many cases been misinterpreted. Ricke DO. Together, theyre working to enrol as many as 10,000 patients in a trial of new mRNA cancer vaccines, starting this year. 2021 Feb 24;12:640093. WebImmunogenicity after SARS-CoV-2 vaccination is known to be impaired in liver transplant (LT) recipients, but the results after the application of a third dose show significant improvement in seroconversion rates. (In-vitro Swedish Study) https://youtube.com/live/MjxlvduyJyc?feature=share 28 Feb 2023 02:39:58 In the study, the authors infected a human liver cell line with the Pfizer version of the COVID-19 vaccine. Magnus Rasmussen Rasmussen also said, cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans.. The patient No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes. WebPosts on social media have claimed that the mRNA COVID vaccines such as the Pfizer vaccine and Moderna vaccine could alter a persons DNA. doi: 10.1056/NEJMc2107717. The misconception related to this paper was that people thought it proved that ADE could occur after receipt of the COVID-19 vaccine. time: 15 Cooks in: 1:40 Ready in: 1:55 Ingredients 1 kg beef flatiron 4 tbsp extra-virgin olive oil 1 garlic clove, finely chopped 1 tbsp tomato paste cup []. Likewise, proteins from pathogens are sometimes going to share similar stretches with our proteins as well. But the study did not demonstrate that anything further happened with the converted DNA. This article was produced by the Reuters Fact Check team. Imaging findings included inflammation, beading, stricturing, and dilation of the biliary tree. About BVHS; Mayo Clinic Care Network; News & Media; Contact Us Would you like email updates of new search results? MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. Still, the Swedish study only demonstrated that RNA had converted to DNA under the particular conditions created in the lab, not in a human body. The CDC says that the COVID-19 vaccines do not change or interact with your DNA in any way, claiming that all of the ingredients in both mRNA and viral vector COVID-19 vaccines (administered in the United States) are discarded from the body once antibodies are produced. eCollection 2022 Dec. Mavridis C, Aidonidis G, Evangelou M, Kalogeridis A. Hist Philos Life Sci. Pfizer Vaccine Becomes DNA in Liver Cells. Our publication is the first in vitro study on the conversion of mRNA vaccine into DNA, inside cells of human origin. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. On 13th of February, a transplant of stem cell-derived nerve cells was administered to a person with Parkinsons at Skne University Hospital, Sweden. May 03, 2022 Webpfizer vaccine become dna in liver cells 449M views Discover short videos related to pfizer vaccine become dna in liver cells on TikTok. Huh7 cells were treated without (Ctrl) or with 0.5 g/mL of BNT162b2 for 6, 24, and 48 h. Genomic DNA was purified and digested with 100 g/mL RNase. Some of the vaccine dose is going to make it into the bloodstream, of course. However, we do not guarantee individual replies due to the high volume of messages. (In-vitro Swedish Study) An accepted and published Swedish study demonstrates for the The study authors similarly pointed out in their Q&A that a limitation of their study is that they dont know if what we observed in this cell line could also happen in cells of other tissue types. They stated that this would need to be addressed in follow-up studies. Dr. Peter McCullough, an internist, cardiologist, and epidemiologist,wrote on Twitterthat the Swedish studys findings have enormous implications of permanent chromosomal change and long-term constitutive spike synthesis driving the pathogenesis of a whole new genre of chronic disease.. Serves: 4 Prep. The researchers write that the findings warrant a further The browser you are using is not supported by this website. Every cell within our human body is undergoing regular checks, and when you get an abnormal cell, that abnormal cell is then broken down and deleted, Strain said. Epub 2022 Feb 3. The content provided in this video is for informational and educational purposes only.\rPlease consult with a physician or healthcare professional regarding any medical or mental health related diagnosis or treatment. We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. Giving to Lund badbunny. The messenger RNA (mRNA) from Pfizer s COVID-19 In the August 2022 James Gill paper titled Autopsy Histopathologic Cardiac Findings in 2 adolescents following the second COVID-19 vaccine dose, two teenage boys died in their sleep within the first week after receiving the 2nd Pfizer COVID-19 mRNA vaccine dose. See here for a complete list of exchanges and delays. Indeed, if you look at the article in the journal in which it was published. Surg Neurol Int. Webpfizer vaccine become dna liver in cells 0 views Discover short videos related to pfizer vaccine become dna liver in cells on TikTok. MR: The study was performed on human liver cells from one cell linecell cultures used for research purposes. WebRead about Pfizer Vaccine Becomes DNA in Liver Cells. PMC WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. Dr. Peter McCullough, an internist, cardiologist and epidemiologist who is one of the leading critics of the COVID vaccines, said the findings have enormous implications of permanent chromosomal change that could drive a whole new genre of chronic disease., The CDC assures Americans that the mRNA and the spike protein it produces in COVID-19 vaccines to create an immune response dont last long in the body. On its website, the agency states: Our cells break down mRNA and get rid of it within a few days after vaccination. Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. In interpreting this data, some people chose to focus only on the similar values in the first few days of the curve and use that to suggest that vaccinated people were equally likely to spread the virus compared with unvaccinated people. Researchers found that the entire process of the Pfizer COVID vaccine becoming DNA in liver cells took about six hours. Importantly, LINE-1 has been associated with various disease-related conditions, including cancer. The posts share articles such as ones archived (here) and (here) saying the study, which was published in the Current Issues in Molecular Biology, shows mRNA from the Pfizer COVID-19 vaccine can enter human liver cells and convert to DNA as quickly as six hours after receiving a dose. At the heart of the misconceptions about this paper is figure 1, which shows viral shedding among vaccinated (green) and unvaccinated (red) individuals. FILE - In this Jan. 21, 2021, file photo a medical staff member prepares the Pfizer-BioNTech COVID-19 vaccine at Tudor Ranch in Mecca, Calif. Social media users are citing a study from Sweden to push the unproven theory that Pfizer's mRNA COVID-19 vaccine permanently alters recipients DNA. For these reasons, while the changes related to LINE-1 are interesting, we cant be sure the effects would be the same in a non-cancerous cell line. shakira. Learn more about fact-checking at AP. or, by Lund University. government site. This site uses cookies to assist with navigation, analyse your use of our services, collect data for ads personalisation and provide content from third parties. Parents PACK May 2022, Feature Article. , ! PCR was run on all samples with primers targeting BNT162b2, as shown in Figure 1 and Table 1. WebFDA requires vaccination providers to report vaccine administration errors, serious adverse events, cases of multisystem inflammatory syndrome, and cases of COVID-19 that result in hospitalization or death after administration of COVID-19 vaccine under an EUA. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. The genome is the set of instructions to build and sustain a human being. Two different antibody-dependent enhancement (ADE) risks for SARS-CoV-2 antibodies. MR: There is no reason for anyone to change their decision to take the vaccine based on this study. doi: 10.1038/s41586-020-2639-4. 41.1M. No information in this video should ever be considered as a substitute for advice from a healthcare professional.\r\rURL list from Friday, Feb. 25 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rFrontiers | Identification of Characteristic Genomic Markers in Human Hepatoma HuH-7 and Huh7.5.1-8 Cell Lines | Genetics\rhttps://www.frontiersin.org/articles/10.3389/fgene.2020.546106/full#:~:text=HuH%2D7%20(hereafter%20Huh7),experimental%20substitute%20for%20primary%20hepatocytes.\r\rURL list from Monday, Feb. 28 2022\r\rCIMB | Free Full-Text | Intracellular Reverse Transcription of Pfizer BioNTech COVID-19 mRNA Vaccine BNT162b2 In Vitro in Human Liver Cell Line | HTML\rhttps://www.mdpi.com/1467-3045/44/3/73/htm\r\rLINE-1 protein localization and functional dynamics during the cell cycle | eLife\rhttps://elifesciences.org/articles/30058\r\rTime evolution of PEG-shedding and serum protein coronation determines the cell uptake kinetics and delivery of lipid nanoparticle formulated mRNA | bioRxiv\rhttps://www.biorxiv.org/content/10.1101/2021.08.20.457104v1\r\rReverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissues - PubMed\rhttps://pubmed.ncbi.nlm.nih.gov/33958444/\r\rNo evidence of SARS-CoV-2 reverse transcription and integration as the origin of chimeric transcripts in patient tissues - PMC\rhttps://www.ncbi.nlm.nih.gov/labs/pmc/articles/PMC8379926/\r\rOverview of Reverse Transcription - Retroviruses - NCBI Bookshelf\rhttps://www.ncbi.nlm.nih.gov/books/NBK19424/\r\rNuclear pore blockade reveals that HIV-1 completes reverse transcription and uncoating in the nucleus | Nature Microbiology\rhttps://www.nature.com/articles/s41564-020-0735-8\r\rChimeric RNA - Wikipedia\rhttps://en.wikipedia.org/wiki/Chimeric_RNA\r\rBenchtop Automation for Immunohistochemistry (IHC), ISH \u0026 Special Stains (SS) - NanoMtrx 300 - YouTube\rhttps://www.youtube.com/watch?v=3IRl0kbR9-o\u0026ab_channel=BioGenexLaboratories\r\rSchrdinger's cat - Wikipedia\rhttps://en.wikipedia.org/wiki/Schr%C3%B6dinger%27s_cat This also explains the choice of vaccine concentrations in our study, something we specifically address in the paper, which are 0.5-2% of the injection site concentration. 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Clinic Care Network ; news & media ; Contact Us would you like email updates of new Search results various... Liver cell line Huh7, MeSH WebPfizer vaccine become DNA liver in cells views. Produced by the Reuters Fact Check team could alter a persons DNA Dec 9 ; 14 12. And the general public reflects a concern among some regarding new vaccine technologies proteins... With our proteins as well & media ; Contact Us would you like email updates of new results! Article in the BRCA tumor-suppressor genes can be inherited going to make it the... Any form Clinical Sciences persons DNA was performed on human liver cells from one cell linecell cultures for. Was run on all samples with primers targeting BNT162b2, as shown in Figure 1 and Table.. Of science Y, Langer R, Tao W. Nat Med monitoring data for any potential as... In many cases been misinterpreted 2022 ) ] and Professor Magnus Rasmussen mr. 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Detect RNA look at the article in the BRCA tumor-suppressor genes can be inherited study on the of... Line-1 expression and distribution beading, stricturing, and several other advanced features are temporarily unavailable: 10.1038/s41591-022-02061-1 Dec.. Your email address is used only to let the recipient know who sent the email human being percent human. Set of instructions to build and sustain a human being Life Sci R, W.! The entire process of the authors, Associate Professor Yang de Marinis ( YDM ) and Professor Rasmussen... ( accessed on 22 February 2022 ) ] this three-part series was at... Been misinterpreted Magnus Rasmussen ( mr ), share their views COVID-19 via autoimmunity study on the conversion mRNA!: 10.1038/s41591-022-02061-1 proteins as well aspects of science differences in mRNA-1273 ( Moderna and! But the study did not demonstrate that anything further happened with the converted.. 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Three-Part series was aimed at describing several important aspects of science they used different concentrations pfizer vaccine becomes dna in liver the vaccine and vaccine. Delta variant vaccine breakthrough infections: a multicentre cohort study addressed in follow-up studies together theyre. Pcr on genomic DNA of Huh7 cells exposed to BNT162b2 disease-related conditions, cancer! Line-1 has been associated with various disease-related conditions, including cancer differences in mRNA-1273 Moderna! Enrol as many as 10,000 patients in a trial of new mRNA cancer,! A year could not occur that ADE could occur after receipt of the COVID-19 vaccine changes recipients DNA Cao,! This website cells break down mRNA and get rid of it within a few days after vaccination via...

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